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    CST Anti-mouse IgG, HRP-linked Antibody

    簡要描述:CST Anti-mouse IgG, HRP-linked Antibody 來自CST公司的7076S,HRP標記馬抗小鼠二抗,可以做WB

    • 產品型號:7076S
    • 廠商性質:代理商
    • 更新時間:2023-12-19
    • 訪  問  量:486

    詳細介紹

    品牌CST貨號7076S
    規格1ml供貨周期一周

    Product Usage Information

    Recommended Antibody Dilutions:

    1:1000–1:3000

    20X LumiGLO® Reagent and 20X Peroxide #7003 1:1K–1:3K

    SignalFireTM ECL Reagent #6883 1:1K–1:3K

    SignalFireTM Plus ECL Reagent #12630 1:5K-1:15K

    SignalFireTM Elite ECL Reagent #12757 1:10K-1:20K

    Storage

    Supplied in 10mM sodium HEPES (pH 7.5), 150 mM NaCl, 2 mg/ml bovine serum albumin (BSA) and 50% glycerol. Store at –20°C. Do not aliquot the antibodies. This product is stable for 60 months when stored at -20C.

    Product Description

    Affinity purified horse anti-mouse IgG (heavy and light chain) antibody is conjugated to horseradish peroxidase(HRP) for chemiluminescent detection.  This product is thoroughly validated with CST primary antibodies and will work optimally with the CST western immunoblotting protocol, ensuring accurate and reproducible results.

    Background

    Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.

      Species Reactivity

      Species reactivity is determined by testing in at least one approved application (e.g., western blot).

      Western Blot Buffer

      IMPORTANT: For western blots, incubate membrane with diluted primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 at 4°C with gentle shaking, overnight.

      Applications Key

      WB: Western Blotting

      Cross-Reactivity Key

      H: human M: mouse R: rat Hm: hamster Mk: monkey Vir: virus Mi: mink C: chicken Dm: D. melanogaster X: Xenopus Z: zebrafish B: bovine Dg: dog Pg: pig Sc: S. cerevisiae Ce: C. elegans Hr: horse GP: Guinea Pig Rab: rabbit All: all species expected


      僅限科研使用

      For Research Use Only. Not for Use in Diagnostic Procedures.
      Applications:

      WB

      Source/Isotype:

      Horse









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